Plasmid

Part:BBa_K4687007:Design

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-02)


pBluescript


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 707
    Illegal XbaI site found at 677
    Illegal SpeI site found at 683
    Illegal PstI site found at 701
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 707
    Illegal SpeI site found at 683
    Illegal PstI site found at 701
    Illegal NotI site found at 669
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 707
    Illegal BamHI site found at 689
    Illegal XhoI site found at 740
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 707
    Illegal XbaI site found at 677
    Illegal SpeI site found at 683
    Illegal PstI site found at 701
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 707
    Illegal XbaI site found at 677
    Illegal SpeI site found at 683
    Illegal PstI site found at 701
    Illegal NgoMIV site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Plasmid vectors introduced the CRISPR components into the cells to ensure that they function properly within the cell. By selecting different plasmid vectors for experiments, the most suitable ones were selected to improve the efficiency of gene editing.


Source

Pragenuclear plasmid in E. coli. As the cloning vector, which originated from the phage subsp.

References